pMIR-9
(Plasmid #25037)

• Depositing Lab: Bob Weinberg
• Publication: Ma et al Nat Cell Biol. 2010 Mar . 12(3):247-56.

Backbone

• Vector backbone: pMIR-report
• Backbone manufacturer: Ambion
• Backbone size w/o insert (bp): 6470
• Vector type: Mammalian Expression, Luciferase
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: miR-9 binding site
• Alt name: microRNA 9
• Species: H. sapiens (human)
• Insert Size (bp): 36
• Entrez Gene: MIR9-1 (a.k.a. MIRN9-1, hsa-mir-9-1, miRNA9-1, mir-9-1)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SpeI (unknown if destroyed)
• 3′ cloning site: HindIII (unknown if destroyed)
• 5′ sequencing primer: Luc-F
• 3′ sequencing primer: M13/pUC Forward

Resource Information

• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Human miR-9 binding site (the reverse complementary sequence of miR-9) was generated by annealing of 2 oligos (5′-CTAGTTCATACAGCTAGATAACCAAAGAGCTCAGCA-3′ (forward) and 5′-AGCTTGCTGAGCTCTTTGGTTATCTAGCTGTATGAA-3′ (reverse)), and ligating into pMIR-report.