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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
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| Plasmid | 25035 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepEGFP-C1
- Backbone size w/o insert (bp) 4770
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsDh5 alpha or XL1-blue
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTNRC6A
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SpeciesH. sapiens (human)
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Insert Size (bp)5130
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GenBank IDNM_014494
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Entrez GeneTNRC6A (a.k.a. CAGH26, FAME6, GW1, GW182, TNRC6)
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Tag
/ Fusion Protein
- EGFP (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (not destroyed)
- 3′ cloning site PstI (not destroyed)
- 5′ sequencing primer EGFP-C
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pT7-EGFP-C1-HsTNRC6A was a gift from Elisa Izaurralde (Addgene plasmid # 25035 ; http://n2t.net/addgene:25035 ; RRID:Addgene_25035) -
For your References section:
The C-terminal domains of human TNRC6A, TNRC6B, and TNRC6C silence bound transcripts independently of Argonaute proteins. Lazzaretti D, Tournier I, Izaurralde E. RNA. 2009 Jun . 15(6):1059-66. 10.1261/rna.1606309 PubMed 19383768
Map uploaded by the depositor.
