pT7-EGFP-C1-HsEDC3
(Plasmid #25032)

• Depositing Lab: Elisa Izaurralde
• Publication: Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 106(51):21591-6.

Backbone

• Vector backbone: pEGFP-C1
• Backbone size w/o insert (bp): 4769
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: Dh5 alpha or XL1-blue
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: EDC3
• Species: H. sapiens (human)
• Insert Size (bp): 1527
• GenBank ID: NM_025083
• Entrez Gene: EDC3 (a.k.a. LSM16, MRT50, YJDC, YJEFN2, hYjeF_N2-15q23)
• Tag / Fusion Protein:
  • EGFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI/BglII (destroyed during cloning)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: EGFP-C
• 3′ sequencing primer: SV40pA-R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry