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Addgene

pKW08-Lx-Int
(Plasmid #25015)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 25015 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMC1M
  • Backbone size w/o insert (bp) 8535
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Hygromycin, 200 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    High copy number in E. coli. Single copy in mycobacteria.
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    pKW08-Lx-Int
  • Insert Size (bp)
    8535

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site N.A (unknown if destroyed)
  • 5′ sequencing primer N/A
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKW08-Lx-Int was a gift from Brian Robertson (Addgene plasmid # 25015 ; http://n2t.net/addgene:25015 ; RRID:Addgene_25015)

  • For your References section:

    Improved mycobacterial tetracycline inducible vectors. Williams KJ, Joyce G, Robertson BD. Plasmid. 2010 Apr 29. ():. 10.1016/j.plasmid.2010.04.003 PubMed 20434484
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