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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 25012 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepMC1M
- Backbone size w/o insert (bp) 7960
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Hygromycin, 200 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsHigh copy number in E. coli. Low copy number in mycobacteria.
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namepKW08-Lx
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Insert Size (bp)7960
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer N/A (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pKW08-Lx was a gift from Brian Robertson (Addgene plasmid # 25012 ; http://n2t.net/addgene:25012 ; RRID:Addgene_25012) -
For your References section:
Improved mycobacterial tetracycline inducible vectors. Williams KJ, Joyce G, Robertson BD. Plasmid. 2010 Apr 29. ():. 10.1016/j.plasmid.2010.04.003 PubMed 20434484
Map uploaded by the depositor.
