Skip to main content
Addgene

pcDNA-SETin-FLAG-HA
(Plasmid #24999)

Print

Full plasmid sequence is not available for this item.

Loading...

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 24999 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone size w/o insert (bp) 6000
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SET
  • Alt name
    2PP2A; IGAAD; TAF-I; I2PP2A; IPP2A2; PHAPII; TAF-IBETA
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1000
  • Mutation
    silent mutations introduced to prevent siRNA targeting:nt57-75 change from GGCCGACGAGACCTCAGAA toCGCGGACGAAACGTCTGAGAA
  • GenBank ID
  • Entrez Gene
    SET (a.k.a. 2PP2A, I2PP2A, IGAAD, IPP2A2, MRD58, PHAPII, TAF-I, TAF-IBETA)
  • Tags / Fusion Proteins
    • FLAG (C terminal on insert)
    • HA (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-rev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA-SETin-FLAG-HA was a gift from Judy Lieberman (Addgene plasmid # 24999 ; http://n2t.net/addgene:24999 ; RRID:Addgene_24999)

  • For your References section:

    The SET complex acts as a barrier to autointegration of HIV-1. Yan N, Cherepanov P, Daigle JE, Engelman A, Lieberman J. PLoS Pathog. 2009 Mar . 5(3):e1000327. 10.1371/journal.ppat.1000327 PubMed 19266025
Related items:
Commonly requested with: