pcDNA-SETin-FLAG-HA
(Plasmid #24999)

• Depositing Lab: Judy Lieberman
• Publication: Yan et al PLoS Pathog. 2009 Mar . 5(3):e1000327.

Backbone

• Vector backbone: pcDNA3.1
• Backbone size w/o insert (bp): 6000
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SET
• Alt name: 2PP2A; IGAAD; TAF-I; I2PP2A; IPP2A2; PHAPII; TAF-IBETA
• Species: H. sapiens (human)
• Insert Size (bp): 1000
• Mutation: silent mutations introduced to prevent siRNA targeting:nt57-75 change from GGCCGACGAGACCTCAGAA toCGCGGACGAAACGTCTGAGAA
• GenBank ID:
• Entrez Gene: SET (a.k.a. 2PP2A, I2PP2A, IGAAD, IPP2A2, MRD58, PHAPII, TAF-I, TAF-IBETA)
• Tags / Fusion Proteins:
  • FLAG (C terminal on insert)
  • HA (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry