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pCMV5b HA Irx5 deltaIrot
(Plasmid #24995)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 24995 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV5b
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Irx5
  • Species
    M. musculus (mouse)
  • Mutation
    Deletion of Iro box (aa 314-327), 19 aa upstream of Iro box, and all downstream of Iro box. Contains aa 1-313.
  • Entrez Gene
    Irx5
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

HA tag is YDVPDYASL.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV5b HA Irx5 deltaIrot was a gift from Benoit Bruneau (Addgene plasmid # 24995 ; http://n2t.net/addgene:24995 ; RRID:Addgene_24995)

  • For your References section:

    The homeodomain transcription factor Irx5 establishes the mouse cardiac ventricular repolarization gradient. Costantini DL, Arruda EP, Agarwal P, Kim KH, Zhu Y, Zhu W, Lebel M, Cheng CW, Park CY, Pierce SA, Guerchicoff A, Pollevick GD, Chan TY, Kabir MG, Cheng SH, Husain M, Antzelevitch C, Srivastava D, Gross GJ, Hui CC, Backx PH, Bruneau BG. Cell. 2005 Oct 21. 123(2):347-58. 10.1016/j.cell.2005.08.004 PubMed 16239150
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