FGF10-luciferase
(Plasmid
#24994)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 24994 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepXP1
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Backbone manufacturerATTC
- Backbone size w/o insert (bp) 6100
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Vector typeMammalian Expression, Luciferase
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameFgf10 promoter
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SpeciesM. musculus (mouse)
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Insert Size (bp)6500
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Entrez GeneFgf10 (a.k.a. AEY17, Fgf-10, Fgf5a, Gsfaey17)
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Tag
/ Fusion Protein
- luciferase (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer N/A
- 3′ sequencing primer LucNRev (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
FGF10-luciferase was a gift from Benoit Bruneau (Addgene plasmid # 24994 ; http://n2t.net/addgene:24994 ; RRID:Addgene_24994) -
For your References section:
Tbx5 is essential for forelimb bud initiation following patterning of the limb field in the mouse embryo. Agarwal P, Wylie JN, Galceran J, Arkhitko O, Li C, Deng C, Grosschedl R, Bruneau BG. Development. 2003 Feb . 130(3):623-33. 10.1242/dev.00191 PubMed 12490567