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Addgene

pGB3
(Plasmid #24993)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 24993 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pPro
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    xynB
  • Species
    C. stercorarium

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (unknown if destroyed)
  • 3′ cloning site XhoI (unknown if destroyed)
  • 5′ sequencing primer unknown
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

DNA encoding the catalytic domain of xynB from C. stercorarium NCIMB 11754 was synthesized and codon-optimized by DNA 2.0. XynB was cloned using an EcoRI site on the 5' end and a BamHI site on the 3' end.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGB3 was a gift from Jay Keasling (Addgene plasmid # 24993 ; http://n2t.net/addgene:24993 ; RRID:Addgene_24993)

  • For your References section:

    Microbial production of fatty-acid-derived fuels and chemicals from plant biomass. Steen EJ, Kang Y, Bokinsky G, Hu Z, Schirmer A, McClure A, Del Cardayre SB, Keasling JD.. Nature. 2010 Jan 28;463(7280):559-62. 10.1038/nature08721 PubMed 20111002
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