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Addgene

pEX-HcRed-hLC3WT
(Plasmid #24991)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 24991 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCXN2
  • Backbone size w/o insert (bp) 6000
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    a wild type human MAP1-LC3B fused with HcRed
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    378
  • Entrez Gene
    MAP1LC3B (a.k.a. ATG8F, LC3B, MAP1A/1BLC3, MAP1LC3B-a)
  • Tag / Fusion Protein
    • HcRed (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Bgl2 (not destroyed)
  • 3′ cloning site SalI/XhoI (destroyed during cloning)
  • 5′ sequencing primer HcRed-C
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEX-HcRed-hLC3WT was a gift from Isei Tanida (Addgene plasmid # 24991 ; http://n2t.net/addgene:24991 ; RRID:Addgene_24991)

  • For your References section:

    Consideration about negative controls for LC3 and expression vectors for four colored fluorescent protein-LC3 negative controls. Tanida I, Yamaji T, Ueno T, Ishiura S, Kominami E, Hanada K. Autophagy. 2008 Jan 1. 4(1):131-4. PubMed 18000393
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