xGoosecoid
(Plasmid
#24977)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 24977 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBS-SK
- Backbone size w/o insert (bp) 3000
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGoosecoid
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SpeciesX. laevis (frog)
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Entrez Genegsc-a (a.k.a. Xgsc, goosecoid)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer T3
- 3′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
For antisense probe: cut with EcoRI, transcribe with T7
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
xGoosecoid was a gift from Edward De Robertis (Addgene plasmid # 24977 ; http://n2t.net/addgene:24977 ; RRID:Addgene_24977) -
For your References section:
Molecular nature of Spemann's organizer: the role of the Xenopus homeobox gene goosecoid. Cho KW, Blumberg B, Steinbeisser H, De Robertis EM. Cell. 1991 Dec 20. 67(6):1111-20. 10.1016/0092-8674(91)90288-A PubMed 1684739