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Addgene

pBS xChordin
(Plasmid #24975)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 24975 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBS-SK
  • Backbone size w/o insert (bp) 3000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Chordin
  • Species
    X. laevis (frog)
  • Insert Size (bp)
    2800
  • Entrez Gene
    chrd.1.S (a.k.a. XELAEV_18029538mg, X-chordin, chd, chrd, chrd.L)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T3
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Partial 2.8 kB clone of the original Xenopus chordin cDNA lacking 3' portion.

For antisense cut with EcoRI, transcribe with T7

For Northern blot probe, cut with EcoRI and XhoI

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBS xChordin was a gift from Edward De Robertis (Addgene plasmid # 24975 ; http://n2t.net/addgene:24975 ; RRID:Addgene_24975)

  • For your References section:

    Xenopus chordin: a novel dorsalizing factor activated by organizer-specific homeobox genes. Sasai Y, Lu B, Steinbeisser H, Geissert D, Gont LK, De Robertis EM. Cell. 1994 Dec 2. 79(5):779-90. 10.1016/0092-8674(94)90068-X PubMed 8001117
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