pCS2 xenopus twisted gastrulation
(Plasmid
#24974)
-
Depositing Lab
-
Publication
-
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 24974 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepCS2
- Backbone size w/o insert (bp) 4100
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nametwisted gastrulation
-
SpeciesM. musculus (mouse)
-
Insert Size (bp)1400
-
Entrez GeneTwsg1 (a.k.a. Tsg, Twg, AW552143, D17Ertd403e, 1810013J15Rik, 9030422N06Rik)
-
Tags
/ Fusion Proteins
- chordin signal peptide (N terminal on insert)
- FLAG (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer SP6
- 3′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
mRNA injection sense: cut with NotI, transcribe with SP6
Contains the Xenopus Chordin signal peptide and the N terminus until Ala41, followed by a Flag tag sequence.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pCS2 xenopus twisted gastrulation was a gift from Edward De Robertis (Addgene plasmid # 24974 ; http://n2t.net/addgene:24974 ; RRID:Addgene_24974) -
For your References section:
Proteolytic cleavage of Chordin as a switch for the dual activities of Twisted gastrulation in BMP signaling. Larrain J, Oelgeschlager M, Ketpura NI, Reversade B, Zakin L, De Robertis EM. Development. 2001 Nov . 128(22):4439-47. PubMed 11714670
Map uploaded by the depositor.
