U6-shRNA pgkCre
(Plasmid #24971)

• Depositing Lab: Tyler Jacks
• Publication: Young et al Proc Natl Acad Sci U S A. 2010 Jun 1. 107(22):10184-9.

Backbone

• Vector backbone: unknown
• Backbone size w/o insert (bp): 7705
• Vector type: Mammalian Expression, Lentiviral, RNAi

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Growth instructions: Stbl3
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: shRNA
• Insert Size (bp): 22

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HpaI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: MSCV-rev

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This vector allows for constitutive Cre and shRNA expression. It does not contain any selection cassette. The shRNA coding oligos have to be cloned into the HpaI and XhoI restriction sites. Oligo design information can be found on the author's map or on the Jacks Lab website http://web.mit.edu/jacks-lab/protocols/pSico.html