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Addgene

pBS xenopus Endodermin (pBS-eddL6)
(Plasmid #24968)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 24968 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBS-SK
  • Backbone size w/o insert (bp) 3000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Endodermin
  • Species
    X. laevis (frog)
  • Entrez Gene
    a2m (a.k.a. Edd, a2mb, endod, endodermin)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

For sense: cut with KpnI, transcribe with T3

For antisense: cut with RI, transcribe with T7

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBS xenopus Endodermin (pBS-eddL6) was a gift from Edward De Robertis (Addgene plasmid # 24968 ; http://n2t.net/addgene:24968 ; RRID:Addgene_24968)

  • For your References section:

    Endoderm induction by the organizer-secreted factors chordin and noggin in Xenopus animal caps. Sasai Y, Lu B, Piccolo S, De Robertis EM. EMBO J. 1996 Sep 2. 15(17):4547-55. PubMed 8887546
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