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Addgene

pCS2 xBtg-X
(Plasmid #24964)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 24964 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCS2
  • Backbone size w/o insert (bp) 4100
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Btg-X
  • Species
    X. laevis (frog)
  • Insert Size (bp)
    2900
  • Entrez Gene
    MGC76328.S (a.k.a. btg-x, xBtg-x)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (destroyed during cloning)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer SP6
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

For sense mRNA cut with NotI, transcribe with SP6

For antisense cut with ClaI, transcribe with T7

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCS2 xBtg-X was a gift from Edward De Robertis (Addgene plasmid # 24964 ; http://n2t.net/addgene:24964 ; RRID:Addgene_24964)

  • For your References section:

    Default neural induction: neuralization of dissociated Xenopus cells is mediated by Ras/MAPK activation. Kuroda H, Fuentealba L, Ikeda A, Reversade B, De Robertis EM. Genes Dev. 2005 May 1. 19(9):1022-7. 10.1101/gad.1306605 PubMed 15879552
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