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Addgene

pcDNA3 Lunatic Fringe
(Plasmid #24962)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 24962 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Lunatic Fringe
  • Species
    X. laevis (frog)
  • Entrez Gene
    lfng

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

A60G amino acid mutation detected in Addgene sequence.

For injection: Cut with XhoI, transcribe with T7 (sense)

For probe: Cut with BamHI, transcribe with SP6 (antisense)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3 Lunatic Fringe was a gift from Edward De Robertis (Addgene plasmid # 24962 ; http://n2t.net/addgene:24962 ; RRID:Addgene_24962)

  • For your References section:

    Exploration of the extracellular space by a large-scale secretion screen in the early Xenopus embryo. Pera EM, Hou S, Strate I, Wessely O, De Robertis EM. Int J Dev Biol. 2005 . 49(7):781-96. 10.1387/ijdb.052003ep PubMed 16172975
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