pcDNA3 xNoggin
(Plasmid
#24960)
-
Depositing Lab
-
Publication
-
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 24960 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepcDNA3
- Backbone size w/o insert (bp) 5400
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameNoggin
-
SpeciesX. laevis (frog)
-
Entrez Genenog.L (a.k.a. XELAEV_18043801mg, Noggin1, nog, nog-A, nog1, noggin-1)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
For antisense: linearize with Kpn1, transcribe with SP6
For sense cut with Xho1, transcribe with T7
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pcDNA3 xNoggin was a gift from Edward De Robertis (Addgene plasmid # 24960 ; http://n2t.net/addgene:24960 ; RRID:Addgene_24960) -
For your References section:
Exploration of the extracellular space by a large-scale secretion screen in the early Xenopus embryo. Pera EM, Hou S, Strate I, Wessely O, De Robertis EM. Int J Dev Biol. 2005 . 49(7):781-96. 10.1387/ijdb.052003ep PubMed 16172975
