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Addgene

pCS2 xBicaudal-C
(Plasmid #24959)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 24959 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCS2
  • Backbone size w/o insert (bp) 4100
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Bicaudal-C
  • Species
    X. laevis (frog)
  • Insert Size (bp)
    3300
  • Entrez Gene
    bicc1-b (a.k.a. bicc1, xBic-C, x-Bic-C, Bicaudal-C)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer SP6
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

For sense mRNA cut with NotI, transcribe with SP6

For antisense cut with BamHI, transcribe with T7

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCS2 xBicaudal-C was a gift from Edward De Robertis (Addgene plasmid # 24959 ; http://n2t.net/addgene:24959 ; RRID:Addgene_24959)

  • For your References section:

    The Xenopus homologue of Bicaudal-C is a localized maternal mRNA that can induce endoderm formation. Wessely O, De Robertis EM. Development. 2000 May . 127(10):2053-62. PubMed 10769230
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