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Addgene

LZRS-GADD45A
(Plasmid #24954)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 24954 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    LZRS
  • Backbone size w/o insert (bp) 11100
  • Vector type
    Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    GADD45A
  • Species
    H. sapiens (human)
  • Entrez Gene
    GADD45A (a.k.a. DDIT1, GADD45)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ sequencing primer TGGATACACGCCGCCCACGTG
  • 3′ sequencing primer ATCGTCGACCACTGTGCTGG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The cloning primers used for GADD45A were GADD45A for, ACGCAAAGCTTGCCACCATGACTTTGGAGGAATTCTCGGCTGGAGAGC; GADD45A rev, ACGCAGCGGCCGCTCACCGTTCAGGGAGATTAATCACTGGAACC.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    LZRS-GADD45A was a gift from Paul Khavari (Addgene plasmid # 24954 ; http://n2t.net/addgene:24954 ; RRID:Addgene_24954)

  • For your References section:

    DNMT1 maintains progenitor function in self-renewing somatic tissue. Sen GL, Reuter JA, Webster DE, Zhu L, Khavari PA. Nature. 2010 Jan 28. 463(7280):563-7. 10.1038/nature08683 PubMed 20081831
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