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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 24951 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepSuper Retro
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Backbone manufacturerOligoEngine
- Backbone size w/o insert (bp) 6349
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Vector typeMammalian Expression, Retroviral, RNAi
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameshRNA DNMT1i targeting the 3′UTR
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SpeciesH. sapiens (human)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer pLXSN-5
- 3′ sequencing primer H1 (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
DNMT1i shRNA oligo seq is - GTATGAGTGGAAATTAAGA
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
shRNA-DNMT1i(3'UTR) was a gift from Paul Khavari (Addgene plasmid # 24951 ; http://n2t.net/addgene:24951 ; RRID:Addgene_24951) -
For your References section:
DNMT1 maintains progenitor function in self-renewing somatic tissue. Sen GL, Reuter JA, Webster DE, Zhu L, Khavari PA. Nature. 2010 Jan 28. 463(7280):563-7. 10.1038/nature08683 PubMed 20081831