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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 24942 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepGL2B
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Backbone manufacturerPromega
- Backbone size w/o insert (bp) 5597
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Vector typeLuciferase
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameIL-10 promoter
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SpeciesM. musculus (mouse)
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Insert Size (bp)1602
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Entrez GeneIl10 (a.k.a. CSIF, If2a, Il-10)
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Tag
/ Fusion Protein
- Luciferase (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer N/A
- 3′ sequencing primer LucNRev (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
There are 3 point mutations in the promoter, but they should not affect activity.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGL2B -1538/+64 was a gift from Stephen Smale (Addgene plasmid # 24942 ; http://n2t.net/addgene:24942 ; RRID:Addgene_24942) -
For your References section:
A prominent role for Sp1 during lipopolysaccharide-mediated induction of the IL-10 promoter in macrophages. Brightbill HD, Plevy SE, Modlin RL, Smale ST. J Immunol. 2000 Feb 15. 164(4):1940-51. PubMed 10657644
