pBabepuro3-p18
(Plasmid
#24935)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 24935 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBabe puro3
- Backbone size w/o insert (bp) 5200
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Vector typeMammalian Expression, Retroviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Stbl3
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Growth instructionsStbl3
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namep18
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SpeciesH. sapiens (human)
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Insert Size (bp)600
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Entrez GeneCDKN2C (a.k.a. INK4C, p18, p18-INK4C)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer pBABE-5
- 3′ sequencing primer pBABE-3 (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byDr Yue Xiong
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBabepuro3-p18 was a gift from Lynda Chin (Addgene plasmid # 24935 ; http://n2t.net/addgene:24935 ; RRID:Addgene_24935) -
For your References section:
Feedback circuit among INK4 tumor suppressors constrains human glioblastoma development. Wiedemeyer R, Brennan C, Heffernan TP, Xiao Y, Mahoney J, Protopopov A, Zheng H, Bignell G, Furnari F, Cavenee WK, Hahn WC, Ichimura K, Collins VP, Chu GC, Stratton MR, Ligon KL, Futreal PA, Chin L. Cancer Cell. 2008 Apr . 13(4):355-64. 10.1016/j.ccr.2008.02.010 PubMed 18394558