MSCVhygro-F-Ezh2-F667I
(Plasmid #24927)

• Depositing Lab: Kai Ge
• Publication: Wang et al Proc Natl Acad Sci U S A. 2010 Apr 20. 107(16):7317-22.

Backbone

• Vector backbone: MSCV hygro
• Backbone size w/o insert (bp): 7000
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Ezh2
• Species: M. musculus (mouse)
• Insert Size (bp): 2200
• Mutation: Phenylanaline(F)667 mutated to Isoleucine(I)
• Entrez Gene: EZH2 (a.k.a. ENX-1, ENX1, EZH2b, KMT6, KMT6A, WVS, WVS2)
• Tag / Fusion Protein:
  • FLAG (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: pLXSN 5'
• 3′ sequencing primer: MSCV-Rev

Resource Information

• A portion of this plasmid was derived from a plasmid made by: MGC clone BC016391
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry