pCMV-myc-LC3 (Atg8)
(Plasmid #24919)

• Depositing Lab: Toren Finkel
• Publication: Lee et al Proc Natl Acad Sci U S A. 2008 Mar 4. 105(9):3374-9.

Backbone

• Vector backbone: pCMV-Myc
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 3800
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: LC3
• Alt name: Atg8
• Alt name: microtubule-associated protein 1 light chain 3
• Species: H. sapiens (human)
• GenBank ID: BC067797
• Entrez Gene: MAP1LC3B (a.k.a. ATG8F, LC3B, MAP1A/1BLC3, MAP1LC3B-a)
• Tag / Fusion Protein:
  • Myc (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: BglII (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: EBV-Rev

Resource Information

• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry