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Addgene

pENTR-fishRab8A[T22N]
(Plasmid #24906)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 24906 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDONR
  • Backbone size w/o insert (bp) 2500
  • Vector type
    Gateway entry vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    fishRab8A
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    624
  • Mutation
    Changed Threonine 22 to Asparagine

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ cloning site Gateway recombination (not destroyed)
  • 3′ cloning site Gateway recombination (not destroyed)
  • 5′ sequencing primer M13F(-20)
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This construct was generated by gene synthesis. There are several nucleotide differences between the insert sequence and Addgene quality control sequence but these do not alter the amino acid sequence.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pENTR-fishRab8A[T22N] was a gift from Maxence Nachury (Addgene plasmid # 24906 ; http://n2t.net/addgene:24906 ; RRID:Addgene_24906)

  • For your References section:

    A core complex of BBS proteins cooperates with the GTPase Rab8 to promote ciliary membrane biogenesis. Nachury MV, Loktev AV, Zhang Q, Westlake CJ, Peranen J, Merdes A, Slusarski DC, Scheller RH, Bazan JF, Sheffield VC, Jackson PK. Cell. 2007 Jun 15. 129(6):1201-13. 10.1016/j.cell.2007.03.053 PubMed 17574030
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