GST-p25
(Plasmid #24896)

• Depositing Lab: Harish Pant
• Publication: Amin et al J Neurosci Res. 2002 Feb 1. 67(3):354-62.

Backbone

• Vector backbone: pGEX-2TK
• Backbone size w/o insert (bp): 4969
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: p25
• Alt name: p35 Truncated
• Species: H. sapiens (human)
• Mutation: N-terminal truncated version of p35, contains aa 99-307
• Entrez Gene: CDK5R1 (a.k.a. CDK5P35, CDK5R, NCK5A, p23, p25, p35, p35nck5a)
• Tag / Fusion Protein:
  • GST (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: M13-Puc_Rev, pGEX5'
• 3′ sequencing primer: pGEX3'

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Li-Huei Tsai

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry