human pirh2-(1-261)
(Plasmid #24878)

• Depositing Lab: Cheryl Arrowsmith
• Publication: Sheng et al Nat Struct Mol Biol. 2008 Dec . 15(12):1334-42.

Backbone

• Vector backbone: pGEX-4T-1
• Backbone size w/o insert (bp): 4969
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Pirh2
• Species: H. sapiens (human)
• Insert Size (bp): 783
• Entrez Gene: RCHY1 (a.k.a. ARNIP, CHIMP, PIRH2, PRO1996, RNF199, ZCHY, ZNF363)
• Tag / Fusion Protein:
  • GST (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: pGEX5'
• 3′ sequencing primer: pGEX3'

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

E. coli strain for expression: BL21DE3

Expression conditions: Pirh2 proteins need Zn2+ to fold properly, so 50 uM of ZnCl2 is added to the growth media and purification buffers.

Induction conditions: 0.5mM-1.0mM IPTG at RT for 3.5-4 hours.