human p53-(355-393)
(Plasmid #24870)

• Depositing Lab: Cheryl Arrowsmith
• Publication: Ayed et al Nat Struct Biol. 2001 Sep . 8(9):756-60.

Backbone

• Vector backbone: pGEX-2TK
• Backbone size w/o insert (bp): 5000
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: p53
• Species: H. sapiens (human)
• Insert Size (bp): 114
• Mutation: Contains aa 355-393.
• Entrez Gene: TP53 (a.k.a. BCC7, BMFS5, LFS1, P53, TRP53)
• Tag / Fusion Protein:
  • GST (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: pGEX5'

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

E.Coli strain for epression: BL21DE3

Induction condition: 0.3 mM IPTG at RT for 3 hours or 15 degrees Celsius, overnight.

NdeI site is inserted at 5'-end of BamHI site on pGEX-2TK vector.