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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 24865 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepET15b
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Backbone manufacturerNovagen
- Backbone size w/o insert (bp) 5700
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namep53
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SpeciesH. sapiens (human)
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Insert Size (bp)1080
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MutationContains aa 1-360.
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Entrez GeneTP53 (a.k.a. BCC7, BMFS5, LFS1, P53, TRP53)
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Tag
/ Fusion Protein
- His (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
E.Coli strain for epression: BL21DE3-pLysS
p53 proteins are expressed better and more stable at low temperature
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
human p53-(1-360) was a gift from Cheryl Arrowsmith (Addgene plasmid # 24865 ; http://n2t.net/addgene:24865 ; RRID:Addgene_24865) -
For your References section:
Latent and active p53 are identical in conformation. Ayed A, Mulder FA, Yi GS, Lu Y, Kay LE, Arrowsmith CH. Nat Struct Biol. 2001 Sep . 8(9):756-60. 10.1038/nsb0901-756 PubMed 11524676