PdarC1
(Plasmid
#24835)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 24835 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCTCON2
- Backbone size w/o insert (bp) 6300
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Vector typeYeast Expression
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Selectable markersTRP1
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePdarC1
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Speciessynthetic
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Insert Size (bp)465
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Tags
/ Fusion Proteins
- HA (N terminal on backbone)
- Myc (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer none (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byCloned by Irwin Chen and David Liu
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
PdarC1 was a gift from David Baker (Addgene plasmid # 24835 ; http://n2t.net/addgene:24835 ; RRID:Addgene_24835) -
For your References section:
A de novo protein binding pair by computational design and directed evolution. Karanicolas J, Corn JE, Chen I, Joachimiak LA, Dym O, Peck SH, Albeck S, Unger T, Hu W, Liu G, Delbecq S, Montelione GT, Spiegel CP, Liu DR, Baker D. Mol Cell. 2011 Apr 22;42(2):250-60. Epub 2011 Mar 31. 10.1016/j.molcel.2011.03.010 PubMed 21458342