Skip to main content
Addgene

mPERK
(Plasmid #24826)

Print

Full plasmid sequence is not available for this item.

Loading...

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 24826 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pLenti CMV/TO Puro DEST (670-1)
  • Backbone manufacturer
    Eric Campeau (Addgene plasmid 17293)
  • Backbone size w/o insert (bp) 9594
  • Vector type
    Mammalian Expression, Lentiviral ; Destination vector
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mPERK
  • Alt name
    Eif2ak3
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Eif2ak3 (a.k.a. Pek, Perk)

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ cloning site Gateway (unknown if destroyed)
  • 3′ cloning site Gateway (unknown if destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer MSCV-rev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mPERK was a gift from Fumihiko Urano (Addgene plasmid # 24826 ; http://n2t.net/addgene:24826 ; RRID:Addgene_24826)

  • For your References section:

    Transcriptional regulation of VEGF-A by the unfolded protein response pathway. Ghosh R, Lipson KL, Sargent KE, Mercurio AM, Hunt JS, Ron D, Urano F. PLoS One. 2010 . 5(3):e9575. 10.1371/journal.pone.0009575 PubMed 20221394
Related items:
Commonly requested with: