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Addgene

3XFlag pCMV5-TOPO TAZ ∂CC
(Plasmid #24816)

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Full plasmid sequence is not available for this item.

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Item Catalog # Description Quantity Price (USD)
Plasmid 24816 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pCMV5-TOPO
  • Backbone size w/o insert (bp) 5000
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TAZ
  • Alt name
    WWTR1
  • Species
    H. sapiens (human)
  • Mutation
    Deletion of amino acids 222-265 (coiled coil domain)
  • Entrez Gene
    WWTR1 (a.k.a. TAZ)
  • Tag / Fusion Protein
    • 3XFlag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer CMV-F
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    3XFlag pCMV5-TOPO TAZ ∂CC was a gift from Jeff Wrana (Addgene plasmid # 24816 ; http://n2t.net/addgene:24816 ; RRID:Addgene_24816)

  • For your References section:

    TAZ controls Smad nucleocytoplasmic shuttling and regulates human embryonic stem-cell self-renewal. Varelas X, Sakuma R, Samavarchi-Tehrani P, Peerani R, Rao BM, Dembowy J, Yaffe MB, Zandstra PW, Wrana JL. Nat Cell Biol. 2008 Jul . 10(7):837-48. 10.1038/ncb1748 PubMed 18568018
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