3XFlag pCMV5-TOPO TAZ (∂393)
(Plasmid
#24812)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 24812 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * | |
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Backbone
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Vector backbonepCMV5-TOPO
- Backbone size w/o insert (bp) 5000
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTAZ
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Alt nameWWTR1
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SpeciesH. sapiens (human)
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Insert Size (bp)1179
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MutationContains ONLY amino acids 1-393. (No PDZ binding domain)
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Entrez GeneWWTR1 (a.k.a. TAZ)
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Tag
/ Fusion Protein
- 3XFlag (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI/HindIII (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
3XFlag pCMV5-TOPO TAZ (∂393) was a gift from Jeff Wrana (Addgene plasmid # 24812 ; http://n2t.net/addgene:24812 ; RRID:Addgene_24812) -
For your References section:
TAZ controls Smad nucleocytoplasmic shuttling and regulates human embryonic stem-cell self-renewal. Varelas X, Sakuma R, Samavarchi-Tehrani P, Peerani R, Rao BM, Dembowy J, Yaffe MB, Zandstra PW, Wrana JL. Nat Cell Biol. 2008 Jul . 10(7):837-48. 10.1038/ncb1748 PubMed 18568018
Map uploaded by the depositor.
