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Addgene

3XFlag DVL2 (∂361-736)
(Plasmid #24806)

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Item Catalog # Description Quantity Price (USD)
Plasmid 24806 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pCMV5
  • Backbone size w/o insert (bp) 4095
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    DVL2
  • Species
    H. sapiens (human)
  • Mutation
    Deletion of aa 361-736
  • Entrez Gene
    DVL2
  • Tag / Fusion Protein
    • 3XFlag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer hGH-PA-R
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    3XFlag DVL2 (∂361-736) was a gift from Jeff Wrana (Addgene plasmid # 24806 ; http://n2t.net/addgene:24806 ; RRID:Addgene_24806)

  • For your References section:

    Regulation of planar cell polarity by Smurf ubiquitin ligases. Narimatsu M, Bose R, Pye M, Zhang L, Miller B, Ching P, Sakuma R, Luga V, Roncari L, Attisano L, Wrana JL. Cell. 2009 Apr 17. 137(2):295-307. 10.1016/j.cell.2009.02.025 PubMed 19379695
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