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pHLEGM3
(Plasmid #24782)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 24782 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFLAME4
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Ptet-Gfp-ASV

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    1. Gfp-LAA obtained from Marian Blokpoel 2. TetR obtained from Ambrose Cheung 3. Ag85a promoter obtained from Edith Machowski

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

1.Blokpoel, M. C. J., R. O'Toole, M. J. Smeulders, and H. D. Williams. 2003. Development and application of unstable GFP variants to kinetic studies of mycobacterial gene expression. J. Microbiol. Methods 54:203-211. [PubMed]

2.

2.Bateman, B. T., N. P. Donegan, T. M. Jarry, M. Palma, and A. L. Cheung. 2001. Evaluation of a tetracycline-inducible promoter in Staphylococcus aureus in vitro and in vivo and its application in demonstrating the role of sigB in microcolony formation. Infect. Immun. 69:7851-7857. [PubMed]

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHLEGM3 was a gift from Tanya Parish (Addgene plasmid # 24782 ; http://n2t.net/addgene:24782 ; RRID:Addgene_24782)

  • For your References section:

    Use of a tetracycline-inducible system for conditional expression in Mycobacterium tuberculosis and Mycobacterium smegmatis. Carroll P, Muttucumaru DG, Parish T. Appl Environ Microbiol. 2005 Jun . 71(6):3077-84. 10.1128/AEM.71.6.3077-3084.2005 PubMed 15933004
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