pINT3
(Plasmid
#24778)
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Purpose(Empty Backbone)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 24778 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepUC
- Backbone size (bp) 5694
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Vector typeIntegrating vector
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Selectable markersGentamicin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pINT3 was a gift from Tanya Parish (Addgene plasmid # 24778 ; http://n2t.net/addgene:24778 ; RRID:Addgene_24778) -
For your References section:
HemZ is essential for heme biosynthesis in Mycobacterium tuberculosis. Parish T, Schaeffer M, Roberts G, Duncan K. Tuberculosis (Edinb). 2005 May . 85(3):197-204. 10.1016/j.tube.2005.01.002 PubMed 15850757