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Addgene

pHYRSF1-02
(Plasmid #24765)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 24765 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRSF-1b (Invitrogen)
  • Backbone size w/o insert (bp) 3793
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NpuDnaE-IntC
  • Alt name
    intein
  • Alt name
    NpuDnaE
  • Insert Size (bp)
    117
  • Tags / Fusion Proteins
    • His (N terminal on backbone)
    • GB1 (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHYRSF1-02 was a gift from Hideo Iwai (Addgene plasmid # 24765 ; http://n2t.net/addgene:24765 ; RRID:Addgene_24765)

  • For your References section:

    Segmental isotopic labeling of multi-domain and fusion proteins by protein trans-splicing in vivo and in vitro. Muona M, Aranko AS, Raulinaitis V, Iwaï H. Nat Protoc. 2010 . 5(3):574-87. 10.1038/nprot.2009.240 PubMed 20203672
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