pET21a-Traptavidin
(Plasmid #24753)

• Depositing Lab: Mark Howarth
• Publication: Chivers et al Nat Methods. 2010 Apr 11. ():.

Backbone

• Vector backbone: pET21a
• Backbone manufacturer: EMD Biosciences
• Backbone size w/o insert (bp): 5443
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Traptavidin
• Species: Streptomyces avidinii
• Insert Size (bp): 405
• Mutation: Streptavidin with 2 amino acid mutations: S52G and R53D
• GenBank ID: GU952124
• Tag / Fusion Protein:
  • His-6 (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7R

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Traptavidin has a lower off-rate and lower on-rate for biotin conjugates, as well as higher mechanical strength and higher thermal stability. It is tetramer, refolded from inclusion bodies.

This particular material is subject to a UK priority patent application (0919102.4) filed on 30th October 2009