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PurposeAddgene QC next-generation sequencing identified retroviral plasmid backbone elements.
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 24747 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepSUPER
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Backbone manufacturerHalo-Bio RNAi Therapeutics
- Backbone size w/o insert (bp) 3176
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Vector typeRetroviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameshRNA-GFP
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SpeciesH. sapiens (human)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer H1
- 3′ sequencing primer T3 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
shRNA oligo sequence is - 5′-GCAAGCTGACCCTGAAGTTC
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p5189 pSUPER-shRNA-GFP was a gift from Peter Howley (Addgene plasmid # 24747 ; http://n2t.net/addgene:24747 ; RRID:Addgene_24747) -
For your References section:
Bromodomain protein 4 mediates the papillomavirus E2 transcriptional activation function. Schweiger MR, You J, Howley PM. J Virol. 2006 May . 80(9):4276-85. 10.1128/JVI.80.9.4276-4285.2006 PubMed 16611886
