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p5188 pSUPER-shRNA-Brd4 (CT)
(Plasmid #24746)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 24746 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSUPERretro
  • Backbone manufacturer
    Halo-Bio RNAi Therapeutics
  • Backbone size w/o insert (bp) 3176
  • Vector type
    Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    shRNA-Brd4 (CT)
  • Species
    H. sapiens (human)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (unknown if destroyed)
  • 3′ cloning site HindIII (unknown if destroyed)
  • 5′ sequencing primer H1
  • 3′ sequencing primer T3
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

shRNA oligo sequence is - 5′-GCGGGAGCAGGAGCGAAGA

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p5188 pSUPER-shRNA-Brd4 (CT) was a gift from Peter Howley (Addgene plasmid # 24746 ; http://n2t.net/addgene:24746 ; RRID:Addgene_24746)

  • For your References section:

    Bromodomain protein 4 mediates the papillomavirus E2 transcriptional activation function. Schweiger MR, You J, Howley PM. J Virol. 2006 May . 80(9):4276-85. 10.1128/JVI.80.9.4276-4285.2006 PubMed 16611886
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