p6042 pcDNA-Xpress-His-USP5
(Plasmid
#24744)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 24744 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA-4C
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5300
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameUSP5
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SpeciesH. sapiens (human)
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Insert Size (bp)2574
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Entrez GeneUSP5 (a.k.a. ISOT)
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Tags
/ Fusion Proteins
- His (N terminal on backbone)
- Xpress (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (unknown if destroyed)
- 3′ cloning site XbaI (unknown if destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p6042 pcDNA-Xpress-His-USP5 was a gift from Peter Howley (Addgene plasmid # 24744 ; http://n2t.net/addgene:24744 ; RRID:Addgene_24744) -
For your References section:
The ubiquitin-specific peptidase USP15 regulates human papillomavirus type 16 E6 protein stability. Vos RM, Altreuter J, White EA, Howley PM. J Virol. 2009 Sep . 83(17):8885-92. 10.1128/JVI.00605-09 PubMed 19553310