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p6112 pSG5-HPV31-d3-12-E8^E2C(HA)
(Plasmid #24743)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 24743 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSG5
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 4141
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    HPV31 d3-12 E8^E2C (HA)
  • Alt name
    E8;E2C
  • Alt name
    E2 repressor protein
  • Species
    HPV31
  • Insert Size (bp)
    484
  • Mutation
    Amino acids 3-12 missing.
  • Tag / Fusion Protein
    • HA tag in hinge region

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site BamHI (unknown if destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer SV40pA-R
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p6112 pSG5-HPV31-d3-12-E8^E2C(HA) was a gift from Peter Howley (Addgene plasmid # 24743 ; http://n2t.net/addgene:24743 ; RRID:Addgene_24743)

  • For your References section:

    NCoR1 Mediates Papillomavirus E8;E2C Transcriptional Repression. Powell ML, Smith JA, Sowa ME, Harper JW, Iftner T, Stubenrauch F, Howley PM. J Virol. 2010 Feb 24. ():. 10.1128/JVI.02390-09 PubMed 20181716
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