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pDONR221-hSOX10-G1238A-C1240T
(Plasmid #24739)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 24739 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDONR221
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 2545
  • Vector type
    Gateway Donor Vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    (sex determining region Y)-box 10
  • Alt name
    Sox10
  • Alt name
    SRY-box10
  • Alt name
    Dom
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1403
  • Mutation
    G replaced with A at position 1238 and C replaced with T at position 1240 resulting in G413D and H414Y mutations.
  • GenBank ID
    BC002824
  • Entrez Gene
    SOX10 (a.k.a. DOM, PCWH, SOX-10, WS2E, WS4, WS4C)

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ cloning site none/attP1 (not destroyed)
  • 3′ cloning site none/attP2 (not destroyed)
  • 5′ sequencing primer M13F
  • 3′ sequencing primer M13R
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDONR221-hSOX10-G1238A-C1240T was a gift from William Pavan (Addgene plasmid # 24739 ; http://n2t.net/addgene:24739 ; RRID:Addgene_24739)

  • For your References section:

    Frequent mutations in the MITF pathway in melanoma. Cronin JC, Wunderlich J, Loftus SK, Prickett TD, Wei X, Ridd K, Vemula S, Burrell AS, Agrawal NS, Lin JC, Banister CE, Buckhaults P, Rosenberg SA, Bastian BC, Pavan WJ, Samuels Y. Pigment Cell Melanoma Res. 2009 Aug . 22(4):435-44. 10.1111/j.1755-148X.2009.00578.x PubMed 19422606
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