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Addgene

pMCV-R17a
(Plasmid #24729)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 24729 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pZErO-2
  • Backbone size w/o insert (bp) 3292
  • Vector type
    Cloning vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Growth instructions
    CCDB Survival
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Full genome of Human Merkel Cell polyomavirus
  • Species
    polyomavirus
  • Insert Size (bp)
    5391
  • GenBank ID
    KF266963.1

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Eco RI (not destroyed)
  • 3′ cloning site Eco RI (not destroyed)
  • 5′ sequencing primer M13 reverse primer
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

genome can be liberated by digestion with EcoRI

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMCV-R17a was a gift from Christopher Buck (Addgene plasmid # 24729 ; http://n2t.net/addgene:24729 ; RRID:Addgene_24729)

  • For your References section:

    Merkel cell polyomavirus and two previously unknown polyomaviruses are chronically shed from human skin. Schowalter RM, Pastrana DV, Pumphrey KA, Moyer AL, Buck CB.. Cell Host Microbe. 2010 Jun 25;7(6):509-15. 10.1016/j.chom.2010.05.006 PubMed 20542254
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