pCMV-Sport6 Baf60b
(Plasmid
#24723)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 24723 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCMV-Sport6
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 4396
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Vector typeMammalian Expression, Bacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameBaf60b
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Alt nameSmarcd2
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Alt nameBruneau Lab Plasmid 203
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SpeciesM. musculus (mouse)
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Entrez GeneSmarcd2 (a.k.a. Baf60b)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SalI (unknown if destroyed)
- 3′ cloning site NotI (unknown if destroyed)
- 5′ sequencing primer SP6, CMV-F
- 3′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
In situ probe: Cut with EcoRV or KpnI, use T7
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCMV-Sport6 Baf60b was a gift from Benoit Bruneau (Addgene plasmid # 24723 ; http://n2t.net/addgene:24723 ; RRID:Addgene_24723) -
For your References section:
Baf60c is essential for function of BAF chromatin remodelling complexes in heart development. Lickert H, Takeuchi JK, Von Both I, Walls JR, McAuliffe F, Adamson SL, Henkelman RM, Wrana JL, Rossant J, Bruneau BG. Nature. 2004 Nov 4. 432(7013):107-12. 10.1038/nature03071 PubMed 15525990