pCRII Topo-Tbx20 (14)
(Plasmid
#24715)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 24715 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCRII-TOPO
- Backbone size w/o insert (bp) 3900
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin and Kanamycin, 100 & 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTbx20
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Alt nameBruneau Lab Plasmid 212
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SpeciesM. musculus (mouse)
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Entrez GeneTbx20 (a.k.a. 9430010M06Rik, AL022859, Tbx12)
Cloning Information
- Cloning method TOPO Cloning
- 5′ sequencing primer T7
- 3′ sequencing primer SP6 (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Mouse Tbx20 from e12.5 mouse heart cDNA was isolated by PCR.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCRII Topo-Tbx20 (14) was a gift from Benoit Bruneau (Addgene plasmid # 24715 ; http://n2t.net/addgene:24715 ; RRID:Addgene_24715)
Map uploaded by the depositor.
