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Addgene

pTracer-HV1
(Plasmid #24710)

Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 24710 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pTracer-CMV2
  • Backbone size w/o insert (bp) 6210
  • Vector type
    Mammalian Expression
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    HV1
  • Alt name
    HVCN1
  • Species
    H. sapiens (human)
  • Entrez Gene
    HVCN1 (a.k.a. HV1, VSOP)
  • Tag / Fusion Protein
    • GFP--NOT a fusion! (C terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that this is NOT a GFP fusion. The GFP is located downstream of the insert on the backbone, but is not actually fused to the HV1.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTracer-HV1 was a gift from Yuriy Kirichok (Addgene plasmid # 24710 ; http://n2t.net/addgene:24710 ; RRID:Addgene_24710)
  • For your References section:

    Acid extrusion from human spermatozoa is mediated by flagellar voltage-gated proton channel. Lishko PV, Botchkina IL, Fedorenko A, Kirichok Y. Cell. 2010 Feb 5. 140(3):327-37. 10.1016/j.cell.2009.12.053 PubMed 20144758