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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 24710 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepTracer-CMV2
- Backbone size w/o insert (bp) 6210
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Vector typeMammalian Expression
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Selectable markersZeocin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameHV1
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Alt nameHVCN1
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SpeciesH. sapiens (human)
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Entrez GeneHVCN1 (a.k.a. HV1, VSOP)
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Tag
/ Fusion Protein
- GFP--NOT a fusion! (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note that this is NOT a GFP fusion. The GFP is located downstream of the insert on the backbone, but is not actually fused to the HV1.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pTracer-HV1 was a gift from Yuriy Kirichok (Addgene plasmid # 24710 ; http://n2t.net/addgene:24710 ; RRID:Addgene_24710) -
For your References section:
Acid extrusion from human spermatozoa is mediated by flagellar voltage-gated proton channel. Lishko PV, Botchkina IL, Fedorenko A, Kirichok Y. Cell. 2010 Feb 5. 140(3):327-37. 10.1016/j.cell.2009.12.053 PubMed 20144758