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Purpose(Empty Backbone)
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 24705 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCI-neo
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Backbone manufacturerInvitrogen
- Backbone size (bp) 5653
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Vector typeMammalian Expression, Bacterial Expression, Yeast Expression ; Xenopus oocyte expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)XL1 Blue
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Growth instructionsAge I XL1-Blue
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
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GenBank IDEF177812
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site MluI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer TATGTAGCTTAGAGACTCCATTCGGGTGTTC (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pUNIV backbone was a gift from Cynthia Czajkowski (Addgene plasmid # 24705 ; http://n2t.net/addgene:24705 ; RRID:Addgene_24705) -
For your References section:
Optimized expression vector for ion channel studies in Xenopus oocytes and mammalian cells using alfalfa mosaic virus. Venkatachalan SP, Bushman JD, Mercado JL, Sancar F, Christopherson KR, Boileau AJ. Pflugers Arch. 2007 Apr . 454(1):155-63. 10.1007/s00424-006-0183-1 PubMed 17146677
