GFAP-Cre
(Plasmid #24704)

• Depositing Lab: Michael Sofroniew
• Publication: Bush et al Neuron. 1999 Jun . 23(2):297-308.

Backbone

• Vector backbone: GFAP-HSV-TK
• Backbone manufacturer: Michael Sofroniew (Addgene Plasmid# 24703)
• Backbone size w/o insert (bp): 18000
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Cre
• Species: Synthetic
• Promoter: GFAP (glial fibrillary acidic protein)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI (not destroyed)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: T7

Resource Information

• Supplemental Documents:
  • GFAP-Cre
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

A modified Cre (including an artificial intron, nuclear localization signal and HSV-TK polyA was derived from pACN (Bunting et al., (1999) Genes Dev 13: 1524-1528) to create GFAP-Cre as described in Garcia et al., (2004) Nat Neurosci. 7:1233-1241.

Adaptors were used to clone Cre into the NotI/SalI sites of the GFAP-HSV-TK plasmid described in Bush et al. (1998) Cell 93:189-201. Details of the original GFAP (glial fibrillary acidic protein) promoter cassette (GFAP-lacZ) can be found in Johnson et al. (1995) Glia 13:174-184.